Microarray Analysis at Single-Molecule Resolution
نویسندگان
چکیده
منابع مشابه
Tracking viral genomes in host cells at single-molecule resolution.
Viral DNA trafficking in cells has large impacts on physiology and disease development. Current methods lack the resolution and accuracy to visualize and quantify viral DNA trafficking at single-molecule resolution. We developed a noninvasive protocol for accurate quantification of viral DNA-genome (vDNA) trafficking in single cells. Ethynyl-modified nucleosides were used to metabolically label...
متن کاملResolving Subcellular miRNA Trafficking and Turnover at Single-Molecule Resolution.
Regulation of microRNA (miRNA) localization and stability is critical for their extensive cytoplasmic RNA silencing activity and emerging nuclear functions. Here, we have developed single-molecule fluorescence-based tools to assess the subcellular trafficking, integrity, and activity of miRNAs. We find that seed-matched RNA targets protect miRNAs against degradation and enhance their nuclear re...
متن کاملHigh-resolution human genome structure by single-molecule analysis.
Variation in genome structure is an important source of human genetic polymorphism: It affects a large proportion of the genome and has a variety of phenotypic consequences relevant to health and disease. In spite of this, human genome structure variation is incompletely characterized due to a lack of approaches for discovering a broad range of structural variants in a global, comprehensive fas...
متن کاملI-37: Establishing High Resolution Genomic Profiles of Single Cells Using Microarray and Next-Generation Sequencing Technologies
The nature and pace of genome mutation is largely unknown. Standard methods to investigate DNA-mutation rely on arraying or sequencing DNA from a population of cells, hence the genetic composition of individual cells is lost and de novo mutation in cell(s) is concealed within the bulk signal. We developed methods based on (SNP-) arraying and next-generation sequencing of single-cell whole-genom...
متن کاملNanometer resolution imaging by single molecule switching
T he fluorescence intensity of single molecules can change dramatically even under constant laser excitation. The phenomenon is frequently called as 'blinking,' and involves molecules switching between high-and low-intensity states (1). In addition to spontaneous blinking, the fluorescence of some special fluor-ophores, such as cyanine dyes and photoactivatable fluorescent proteins, can be swit...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: IEEE Transactions on NanoBioscience
سال: 2010
ISSN: 1536-1241
DOI: 10.1109/tnb.2010.2040627